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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Immunology and Serology | 5-10% | - Immune system principles and components - Immunologic testing methodologies - Infectious disease serology - Autoimmune and immunodeficiency disorders |
| Laboratory Operations | 5-10% | - Quality control and quality assurance - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance - Pre-analytical, analytical, and post-analytical processes |
| Microbiology | 15-20% | - Bacteriology and antimicrobial susceptibility - Identification of pathogenic microorganisms - Mycology, parasitology, and virology - Specimen collection and culture techniques |
| Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Endocrinology and therapeutic drug monitoring - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests |
| Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Transfusion reactions and adverse events - Blood group systems and antigens - Antibody detection and identification - Compatibility testing |
| Urinalysis and Other Body Fluids | 5-10% | - Urine formation and composition - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis - Semen and amniotic fluid testing |
| Hematology | 20-25% | - Routine and automated hematology procedures - White blood cell morphology and diseases - Hemostasis and coagulation testing - Red blood cell physiology and disorders |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. 21 CFR 606.65 states, "Supplies and reagents shall be used in a manner consistent with instructions provided by the manufacturer." The correct answer is A. The reagents must be used according to the manufacturer's instructions for use. If the instructions say one or two drops may be used and the facility procedures also allow one or two drops, then there is no problem with the staff members choosing to use two drops.
While equipment must be calibrated, it does not generally influence the number of drops used.
Staff members often ask co-workers and colleagues from other facilities how to perform tasks, but it is the manufacturer's instructions for the operation of equipment and the use of reagents that must be followed.
A laboratory employee who is performing an internal audit of routine ABO and Rh typing procedures notes that a technologist places two drops of Anti-D in a tube, centrifuges the tube for 20 seconds, and reads the reaction using a magnifying mirror. When questioned about the procedure, the technologist indicates that most staff use two drops of Anti-D reagent because the reactions are stronger with two drops. In addition to reviewing the facility procedure manual, what should be done to ensure regulatory compliance?
A) Check the calibration records for the centrifuge.
B) Review the anti-D manufacturer's current instructions for use.
C) Call a colleague at another institution to ask what they do.
2. Convert the following temperature from Celsius to Fahrenheit
-4 degrees C
A) 14 degrees F
B) 39.2 degrees F
C) 27 degrees F
D) 24.8 degrees F
3. Chain-of-Custody procedures must be followed for
A) blood specimens for alcohol level determination
B) Throat swabs of groups A beta streptococcus screening
C) Therapeutic drug threshold determinations
D) routine urinalysis for glucose and ketones
4. What is the CORRECT blood-to-anticoagulant ratio for coagulations tests?
A) 4:1
B) 9:1
C) 5:1
D) 10:1
5. Thyroid-stimulating immunoglobulins, or TSI's, are IgG antibodies that can bind to thyrotropin (TSH) receptors on the thyroid gland. TSIs mimic the action of TSH, causing excess secretion of thyroxine and triiodothyronine. The TSI level is abnormally high in persons with hyperthyroidism due to Graves' disease.
Which of the following is an autoantibody that binds to TSH receptor sites on thyroid cell membranes preventing thyroid-stimulating hormone binding?
A) Thyroxin-binding globulins
B) Antithyroglobulin
C) Lupus erythematosus
D) Antimicrosomal antibodies
E) Thyroid-stimulating immunoglobulins
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: D | Question # 3 Answer: A | Question # 4 Answer: B | Question # 5 Answer: E |
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